Ceramics-Silikáty 57 (3) 201-209 (2013)

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF SOL–GEL DERIVED NANOMATERIAL IN THE TERNARY SYSTEM 64 % SiO₂ - 31 % CaO - 5 % P₂O₅ AS A BIOACTIVE GLASS: IN VITRO STUDY

In this study, we performed a new bioactive glass formulation with the molar composition 64 % SiO₂ - 31 % CaO - 5 % P₂O₅ by the sol-gel method. Sol-gel derived bioglass material was produced in nanopowder using planetary milling machine, followed by sintering at 700°C, for applications as bioactive material in bioactive scaffolds or in orthopaedic. The obtained material was evaluated by X-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC) analyses, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and nitrogen adsorption pore size. The biocompatibility evaluation of the formed glass was assessed through in vitro cell culture by evaluation of alkaline phosphatase activity of osteoblasts and immersion studies in simulated body fluid (SBF) for different time intervals while monitoring the pH changes and the concentration of calcium, phosphorus and silicon in the SBF medium as key factors in the rapid bonding of this bioactive glass to bone tissue as a high bioactive glass. The present investigation revealed that the sol-gel derived ternary bioglass system has the ability to support the growth of human fetal osteoblastic cells (hFOB 1.19). Finally, this material proved to be non-toxic and compatible for the proposed work in segmental defects in the goat model in vivo.

Keywords: Bioactive glass, Sol gel, In vitro, SBF, Osteoblastic cells